Abbe’s Refractometer Operational SOP

1.0 OBJECTIVE:

To lay down the operating procedure of Abbe’s Refractometer.

2.0 SCOPE:

This shall be applicable to the Abbe’s Refractometer of Quality Control Instrument Laboratory.

3.0 RESPONSIBILITY:

3.1 Technical Assistant/ Helper.

3.2 Q.C Analyst.

4.0 ACCOUNTABILITY:

4.1 Manager-Quality Control.

5.0 MATERIAL AND EQUIPMENT:

5.1 Abbe’s Refractometer.

6.0 PROCEDURE:

6.1 Ensure that the instrument is clean. Follow the SOP.

6.2 Ensure that all the parts including eye lens, prisms and mirror are clean before operation.

6.3 Place the instrument on a table where adequate light can be reflected through the mirror.

6.4 Open the prism lock and place few drops of liquid testing sample at the temperature specified.

6.5 Allow the liquid to smear on the lower prism surface quickly close the prism lock.

6.6 Adjust the eye piece cross wire with a knob by rotating it in a clockwise or anti-clockwise direction so that the sharp demarcation line passes through its center.

6.7 Sharp demarcation is produced in the form of a circle having half-light & half-dark portion.

6.8 A knob provided just above the prism can do fine adjustment.

6.9 Note down the reading from the scale with the help of the magnified lens of the instrument.

6.10 Use thermometer and water jacket with tubes provided in the instrument whenever it is required.

6.11 Clean the instrument and keep under box when not in use.

7.0 REVISION LOG:

Revision No.	Effective Date	Reason
00		New SOP

8.0 REFERENCES:

8.1 Not Applicable.

9.0 ANNEXURES:

9.1 Not Applicable.

10.0 ABBREVIATIONS:

Abbreviation	Expanded Form
SOP	Standard operating procedure
&	And
No.	Number
Ltd.	Limited
Q.C	Quality control
QCE_O	Quality control equipment operation
F	Format