Microscope Calibration SOP

1.0 OBJECTIVE:

To lay down the calibration procedure of Microscope.

2.0 SCOPE:

This shall be applicable to the Microscope of Microbiology Laboratory.

3.0 RESPONSIBILITY:

3.1 Technical Assistant/ Helper.

3.2 Q.C Analyst/ Production Pharmacist.

4.0 ACCOUNTABILITY:

4.1 Manager-Quality Control/ Manager-Production.

5.0 MATERIAL AND EQUIPMENT:

5.1 Microscope.

6.0 PROCEDURE:

6.1 Operate the microscope according to the SOP No. BM/QCE_O/SOP016-00.

6.2 Place the stage micrometer (least count 0.01mm) on the slide holder stage.

6.3 Replace the eye piece of microscope with the oculometer.

6.4 Fix the first mark of oculometer with the first mark of stage micrometer using different magnifying lens (10x, 40x and 100x).

6.5 Calculate the least count oculometer using formula given below for each (x) of objective.

Least count of oculometer (μm) = ______No. of marks of stage micrometer x 0.01 x1000_______

No. of marks of oculometer matched with stage micrometer.

6.6 Record result as per annexure-1.

6.7 Record the result on the “Calibration Record”, “Calibration Log Book” & paste the “Calibration Status Sticker” on Microscope.

6.8 Frequency:

6.8.1 Yearly.

7.0 REVISION LOG:

Revision No.	Effective Date	Reason
00		New SOP

8.0 REFERENCES:

8.1 Not Applicable.

9.0 ANNEXURES:

Annexure 1: Calibration record of Microscope.

Annexure: 1

Calibration record of Microscope

CALIBRATION RECORD OF MICROSCOPE

Calibration date	Date of last calibration done		Next due for calibration

INSTRUMENT DETAILS:
Name of equipment		Frequency
Microscope		Yearly

Sr. #	Objective used	No. of marks of oculometer matched with stage micrometer	No. of marks of micrometer matched with oculometer	Least count of oculometer (μm)

Conclusion: Satisfactory/ Not satisfactory.

Calibrated by/date	Checked by/date

10.0 ABBREVIATIONS:

Abbreviation	Expanded Form
SOP	Standard operating procedure
&	And
No.	Number
Ltd.	Limited
Q.C	Quality control
Q.A	Quality assurance
QCE_B	Quality control equipment calibration
F	Format
mm	Millimeter
μm	Micrometer