Title: Standard operating procedure for determination
of refractive index by using Refractometer.
1.0
OBJECTIVE:
To lay down the procedure for analysis
on Refractometer.
2.0
SCOPE:
This shall be applicable to the Refractometer of Quality
Control Instrument Lab.
3.0
RESPONSIBILITY:
3.1
Technical Assistant/ Helper.
3.2
Q.C Analyst.
4.0
ACCOUNTABILITY:
4.1
Manager-Quality Control.
4.2
Head of the Q.C Department.
5.0
MATERIAL AND EQUIPMENT:
5.1
Refractometer.
5.2
Glassware (according to requirement).
5.3
Sample.
6.0
PROCEDURE:
6.1
Ensure that the area is clean.
6.2
Switch on the main power.
6.3
Set the temperature.
6.4
Open the prism and place approx. 2 drops of sample on the
bottom prism.
6.5
Use care not to touch the prism surface with the dropper.
6.6
Close the prism carefully & place the lamp close to
prism.
6.7
Adjust the eye piece cross wire with a knob by rotating it
in a clockwise and anti-clockwise direction. So that the sharp demarcation line
passes through its centre.
6.8
Sharp demarcation is produced in the form of circle having
half light and half dark portion.
6.9
A knob can be adjusted to a fine adjustment.
6.10 Note down the reading from the scale
with the help of the magnified lens of the instrument.
7.0
REVISION LOG:
|
Revision No. |
Effective Date |
Reason |
|
00 |
|
New
SOP |
8.0
REFERENCES:
8.1
The British Pharmacopoeia. Vol V., Official Monograph /Refractive index: 2015, pp. 244.
9.0
ANNEXURES:
Annexure
1: Observations of Refractometer.
Annexure: 1
Observations of Refractometer
Apparatus:
_____________________
Sample
solution: _________________
Room
temperature: ______________
|
Sr.
# |
Temperature
(oC) |
Refractive
index (n) |
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Remarks:
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