Standard operating procedure of taking absorbance or transmittance reading on UV/VIS Spectrophotometer.

1.0       OBJECTIVE:

To lay down the procedure for analysis on UV/VIS Spectrophotometer.

2.0       SCOPE:

This shall be applicable for analysis on UV/VIS Spectrophotometer of Quality Control Instrument Lab.

3.0       RESPONSIBILITY:

3.1        Technical Assistant.

3.2        Q.C Analyst.

4.0       ACCOUNTABILITY:

4.1        Manager-Quality Control.

4.2        Head of the Q.C Department.

5.0       MATERIAL AND EQUIPMENT:

5.1        UV/VIS Spectrophotometer.

5.2        Blank solution.

5.3        Sample solution.

6.0       PROCEDURE:

6.1        Ensure that the area is clean.

6.2        Switch on the main power.

6.3        Switch on the instrument.

6.4        Allow to stabilize for 15min.

6.5        Set the desired wavelength with knob in increasing order.

6.6        Keep the sensitivity switch at the highest position.

6.7        Put the mode selector at %T position.

6.8        Set the filter wheel as per the wavelength.

6.9        Adjust zero percent transmittance with the help of set zero control.

6.10    Rinse reference cuvette and sample cuvette with blank solution 2 to 3 times.

6.11    Adjust 100% T with the help of % knob.

6.12    Remove the cuvette and drain out the blank solution from it. Rinse it with sample 2 to 3 times and filled with sample.

6.13    Clean the outer surface of the reference cuvette with tissue paper.

6.14    Place the cuvette into the sample holder and cover the cuvette compartment.

6.15    The reading show on the data position is the absorbance or %T of the sample.

6.16    Record the results and make entry in instrument usage log book.